Life Sciences

Microscopy

There is always a demand for higher resolution, faster image acquisition and lower price points in Life Sciences. For multi-photon imaging, our fixed wavelength, ultrashort pulse lasers can excite a broad range of fluorescent dyes at the sample plane without damaging the biological sample, while also generating sufficient non-linear excitation within the sample. Additionally, these sources can be used for label free imaging of certain biological structures via a second harmonic generation response.

2-Single Plane Illumination Microscopy (Lightsheet Microscopy)

This technique is used to image intact organs, embryos and organisms by illuminating samples across a whole plane (or sheet).

 

By generating this sheet of light, the optical power is spread across the whole image, reducing photo-damage and stresses induced on living samples. Additionally, the excellent optical sectioning capability increases the SNR and creates images with higher contrast, when compared against confocal microscopy.

 

The Chromacity 1040 is an ideal source for 2-photon lightsheet microscopy, as its high average power and short pulse duration can help deliver cutting-edge images.

Multi-photon Optogenetics

In optogenetics there is a drive to study increasingly larger groups of neurons, and to image deeper into live brain tissue using multi-photon imaging techniques .

 

The Chromacity 1040 laser is an ideal source for 2-photon microscopy. It provides the required excitation and delivers four key technical benefits:

  • Allows time resolved measurements to be taken
  • Enables the capture of images at greater sample depths
  • High power allows the 1040 to be combined with wide-field techniques to image groups of neurons
  • Reduces photobleaching and photothermal degradation, allowing in vivo experiments to be more accessible

 

Our ultrafast lasers offer the requisite power and pulse duration to perform multi-neuron studies while also providing reliability that allows you to focus more on the imaging and less on the laser.

Second Harmonic Generation Microscopy

For label free imaging, SHG microscopy is an important capability which can reveal the structural organization and molecular orientation within non-centrosymmetric tissue structures.  Studies of crystalized bio-molecules such as starch, collagen and myosin, or fibrous structures such as tendons and muscles can be readily performed.

 

The high average power and short femtosecond pulse durations from the low-cost, easy-to-use Chromacity 1040 make it the ideal laser source to generate SHG images at depth.

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