This technique is used to image intact organs, embryos and organisms by illuminating samples across a whole plane (or sheet).
By generating this sheet of light, the optical power is spread across the whole image, reducing photo-damage and stresses induced on living samples. Additionally, the excellent optical sectioning capability increases the SNR and creates images with higher contrast, when compared against confocal microscopy.
The Chromacity 1040 is an ideal source for 2-photon lightsheet microscopy, as its high average power and short pulse duration can help deliver cutting-edge images.
In optogenetics there is a drive to study increasingly larger groups of neurons, and to image deeper into live brain tissue using multi-photon imaging techniques .
The Chromacity 1040 laser is an ideal source for 2-photon microscopy. It provides the required excitation and delivers four key technical benefits:
Our ultrafast lasers offer the requisite power and pulse duration to perform multi-neuron studies while also providing reliability that allows you to focus more on the imaging and less on the laser.
For label free imaging, SHG microscopy is an important capability which can reveal the structural organization and molecular orientation within non-centrosymmetric tissue structures. Studies of crystalized bio-molecules such as starch, collagen and myosin, or fibrous structures such as tendons and muscles can be readily performed.
The high average power and short femtosecond pulse durations from the low-cost, easy-to-use Chromacity 1040 make it the ideal laser source to generate SHG images at depth.