Topic: Multiphoton imaging using next generation indicators
Recorded: Wed 30th June 2021
Ti:Sapphire systems are widely regarded as the preferred tunable light source for multiphoton imaging, however, they are prohibitively expensive and fall short in sufficient powers for new ultrafast two-photon microscopy modalities.
There is a step-change within the two-photon imaging community to resort to fixed wavelength femtosecond sources (centred around 920 – 1040 nm) which rival the tunability of the Ti:Sapphire, but with higher and more stable average powers.
Dr Manuel Mohr from Stanford University, demonstrates this point during a webinar presentation on jYCaMP: an optimised calcium indicator for two-photon imaging at fiber laser wavelengths. The webinar was co-hosted in association with the British Neuroscience Society (BNA), Scientifica and Chromacity.
Discover how the Chromacity 1040 nm femtosecond laser compares with legacy systems like the Ti:Sapphire in relation to cost, reliability and performance.